Antibacterial and cytotoxic effect of honey mediated copper nanoparticles synthesized using ultrasonic assistance

Nur Afini, I. and Shameli, Kamyar and Wong, Magdelyn Mei Theing * and Teow, Sin Yeang * and Chew, Jactty * and Siti Nur Amalina, M. S. (2019) Antibacterial and cytotoxic effect of honey mediated copper nanoparticles synthesized using ultrasonic assistance. Materials Science and Engineering: C, 104. p. 109899. ISSN 09284931

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Abstract

In this study, a comparative study of effect using honey on copper nanoparticles (Cu-NPs) via simple, environmentally friendly process and inexpensive route was reported. Honey and ascorbic acid act as stabilizing and reducing agents with the assistance of sonochemical method. The products were characterized using UV–visible (UV–vis) spectroscopy, X-Ray Diffraction (XRD), High-Resolution Transmission Electron Microscopy (HRTEM), Field-Emission Scanning Electron Microscopy (FESEM) and Fourier Transform Infrared (FTIR) spectroscopy. The reddish brown colour demonstrated the formation of Cu-NPs and UV–visible proved the plasmon resonance of Cu-NPs. XRD also confirmed a highly pure Cu-NPs obtained with absence of copper oxide in which the structure is crystalline. The spherical size of the Cu-NPs was acquire in the presence of honey which is 3.68 ± 0.78 nm with narrow particle distribution. The antibacterial activity was seen against gram-positive and gram-negative bacteria which are Enterococcus faecalis (E. faecalis) and Escherichia coli (E. coli). At higher concentration of Cu-NPs, they were more effective in killing both bacteria. The Cu-NPs without and with honey exhibited toxicities toward normal and cancerous cells. However, Cu-NPs without honey showed more potent killing activity against normal and cancer cells.

Item Type:	Article
Uncontrolled Keywords:	Antibacterial; Cytotoxicity assay; Copper nanoparticle; Honey mediated; Sonochemical
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RM Therapeutics. Pharmacology
Divisions:	Others > Non Sunway Academics Sunway University > Faculty of Engineering and Technology [formerly School of Engineering and Technology until 14 November 2025; School of Science and Technology until 2020] > Dept. Biological Sciences moved to SMLS wef 2021 Sunway University > Faculty of Medical and Life Sciences [formerly School of Medical and Life Sciences until 14 March 2025; School of Healthcare and Medical Sciences until 2020] > Sir Jeffrey Cheah Sunway Medical School > Department of Clinical medicine and Surgery [formerly Department of Medical Sciences until 21 November 2024]
Depositing User:	Dr Janaki Sinnasamy
Related URLs:	Author Author
Date Deposited:	28 Nov 2019 02:20
Last Modified:	28 Nov 2019 02:20
URI:	http://repository.sunway.edu.my/id/eprint/1151

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